Biochemical oxygen demand (BOD) (5 days, 20 ° C), is the amount of dissolved oxygen used by microorganisms in the biological process of metabolizing organic matter in water. The more organic matter there is (e.g., in sewage and polluted bodies of water), the greater the BOD; and the greater the BOD, the lower the amount of dissolved oxygen available for higher animals such as fishes. The BOD is therefore a reliable gauge of the organic pollution of a body of water. One of the main reasons for treating wastewater prior to its discharge into a water resource is to lower its BOD—i.e., reduce its need of oxygen and thereby lessen its demand from the streams, lakes, rivers, or estuaries into which it is released.